klb (R&D Systems)

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R&D Systems klb
Klb, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+%CE%B2+klotho/Human+Klotho+beta+Antibody/pm41752116-173-8-14
Average 93 stars, based on 14 article reviews

klb - by Bioz Stars, 2026-09

93/100 stars

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Binding Assay:

Article Title: Genetic fusion of human FGF21 to a synthetic polypeptide improves pharmacokinetics and pharmacodynamics in a mouse model of obesity.
Article Snippet: .. Binding affinities of FGF21 and PsTag fusion proteins to human β-klotho (58 890 KB, R&D) were examined by direct binding ELISA. .. Human β-klotho (25 ng per well) was coated in a 96-well ELISA plate (Costar; SigmaAldrich, St. Louis, MO, USA), and various concentrations of FGF21 and PsTag fusion proteins were added into the precoated plate.

Article Title: Genetic fusion of human FGF21 to a synthetic polypeptide improves pharmacokinetics and pharmacodynamics in a mouse model of obesity
Article Snippet: .. Here binding activities of FGF21 and PsTag600‐FGF21 were determined by measuring the EC 50 for binding to human β‐klotho (58890KB, R&D) in a direct binding ELISA. .. Human β‐klotho (25 ng per well) was coated in 96‐well ELISA plate (Costar, USA), and various concentrations of FGF21 and PsTag fusion proteins were added to the pre‐coated plate.

Enzyme-linked Immunosorbent Assay:

Injection:

Article Title: Heating-mediated purification of active FGF21 and structure-based design of its variant with enhanced potency.
Article Snippet: FGF21s were covalently immobilized on research-grade CM5 sensor chip (Cytiva) using amine coupling kit (cat# BR100050; Cytiva) at surface densities of approximately 700–900 response units (RU). .. Human β-Klotho (cat# 5889-KB-050; R&D Systems) was diluted as 2.5-fold series (250, 100, 40, 16, 6.4, 2.56, 1.024, and 0 nM) in running buffer and injected over the sensor chip at a flow rate of 30 μl/min. .. Contact and dissociation was monitored for 300 s. Between each cycle, the chip surface was regenerated by a buffer consisting of 10 mM Glycine–HCl (pH 1.5) for 30 s. The data were processed with the Biacore Insight Evaluation software (GE Healthcare).

Article Title: Heating-mediated purification of active FGF21 and structure-based design of its variant with enhanced potency
Article Snippet: FGF21s were covalently immobilized on research-grade CM5 sensor chip (Cytiva) using amine coupling kit (cat# BR100050; Cytiva) at surface densities of approximately 700–900 response units (RU). .. Human β-Klotho (cat# 5889-KB-050; R&D Systems) was diluted as 2.5-fold series (250, 100, 40, 16, 6.4, 2.56, 1.024, and 0 nM) in running buffer and injected over the sensor chip at a flow rate of 30 μl/min. .. Contact and dissociation was monitored for 300 s. Between each cycle, the chip surface was regenerated by a buffer consisting of 10 mM Glycine–HCl (pH 1.5) for 30 s. The data were processed with the Biacore Insight Evaluation software (GE Healthcare).

other:

Article Title: Genetic fusion of human FGF21 to a synthetic polypeptide improves pharmacokinetics and pharmacodynamics in a mouse model of obesity
Article Snippet: The bound protein complexes were detected by HRP‐labelled FGF21 antibody for each concentration; FGF21 antibody was pursued from Abcam and labelled by HRP labelling kit (Thermo Fisher Scientific Inc.).

Concentration Assay:

Article Title: FGF21 mimetic antibodies and uses thereof
Article Snippet: All samples and reagents were diluted in 10× kinetics buffer (Forte Bio cat #18-1092) with PBS buffer with a 1/10 (v/v) ratio. .. Human β-klotho (R and D Systems 5889-KB-050) was diluted with kinetic buffer to the desired concentration (5 ug/ml). .. Human β-klotho was loaded onto an anti-his sensor (Forte Bio, cat #18-5114) for 20 seconds, then antibody 1 was loaded for 400 seconds onto the sensor until saturation conditions were reached (200 nM).

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